P08 - Role of TRPC6 channels in hippocampal function and plasticity
The aim of this project is the analysis of TRPC6 channel function in dentate gyrus granule cells. In mouse hippocampal slices we will study the role of TRPC6 for slow synaptic transmission mediated by group I mGluRs. We will determine the contribution of TRPC6 to neuronal Ca2+ signaling with emphasis on the regulation of intracellular Ca2+ store homeostasis. We will analyze newly generated genetic mouse models by using two-photon imaging and patch-clamp recordings in situ and in vivo, to explore the role of TRPC6 for hippocampal function, especially concerning adult neurogenesis and spatial memory formation.
A) Images from the Allen Brain Atlas demonstrate that of all brain areas, the dentate gyrus (DG) in the hippocampus shows the highest expression of the Trpc6 gene.
B) Electrophysiological recordings and Ca2+ imaging are performed simultaneously in acute brain slices. Inset: Transmitted light image of a patch-clamp-pipette attached to the soma of a single granule cell in the dentate gyrus. Large image: Confocal fluorescence image of a granule cell filled with a Ca2+ -sensitive fluorescent dye.