P07 - TRP channels in the regulation of presynaptic Ca2+-dynamics
Transient receptor potential channels (TRP-channels) are able to form homo- and/or heteromeric non-selective cation channels, causing Ca2+-influx into the cell. Yet, the role of TRPC-channels in synaptic transmission has remained poorly understood. In the upcoming funding period, we will investigate molecular mechanisms of TRPC-channel activation and its impact on neuronal signaling. For this, we will use single, double, as well as triple TRPC knock out mice in combination with high-resolution electrophysiological recordings from hippocampal neurons in vitro and in situ, in order to identify functionally relevant subunit compositions of synaptic TRPC channels, and to delineate their activation and regulation by interacting proteins.